Studies for protection against cardiovascular disease and diabetes complications


Diabetic dbb mice were used for studies of protection against diabetes, nephropathy and wound healing in vivo after treatments with CoQ, carnosine and AX. In comparison to wild type mice itt was found that diabetic mice at 8 months of age exhibited loss of sensation, hypoalgesia, decreased mechanical hyperalgesia, cold allodynia and decrease sciatic nerve conduction velocity. Daily CoQ treatment during 6 months improved these pathological conditions to a large extent. The histochemical study showed a significant reduction in the proportion of medium-sized neuron profiles. The study on mice was followed up by a study on humans investigating the preventive effect of 3 months treatment with carnosine and CoQ on the development of diabetes and its complications. Recruitment of 40 prediabetic and 40 type 2 diabetic patients without significant complications. The investigations so far performed showed promising results and included as biomarkers serum concentrations of coenzyme Q10 and vitamin E, hydrogen peroxide generation status. Reactive oxygen species (ROS) was determined by free oxygen radicals testing (FORT). A large number of relevant urinary and serum biomarkers, and waist index were determined using with established methods. Diabetic peripheral sensory neuropathy was assessed, and subjects with excessive consumption of alcohol, or treatment with B12, as well as those with a history of myocardial infarction and renal failure were excluded from the study.